In this experiment, we aimed to extract caffeine from the tea leaves in the tea bags provided beginning with a solid-liquid extraction method and then a liquid-liquid extraction. Added 4.8g of Calcium Chloride and 125mL of water over the tea leaves. A wood stick served as a boiling stick to prevent superheating. organic and inorganic compounds. We added some additional hexane to help transfer and wash the crystals through the vacuum filtration. - Tea leaves 2. (2016, Jun 21). Placed 9-10g of tea leaves in a 400mL beaker. The key to extraction is creating a An extraction is taking place each time coffee or tea is made. The concentration of the solutes in the organic layer contributes to the fact that it is below the aqueous layer. By introducing of a basic sat like sodium carbonate to the solution, gallic acid will be converted into an inorganic, phenol salt that is insoluble in methylene chloride but highly soluble in water. A. The remaining solid would then be pure caffeine. Caffeine is a base which can react with acids to form salts. 6.) and our caffeine content in grams was close to the USDA average for ground coffee. Max. Morrison, Robert Thornton., et al. apparatus, The apparatus filtered slower than expected. Caffeine is water soluble but so are some tannins and gallic acid which is formed in the process of boiling tea leaves. Safety Lab coat, goggles, gloves. Minutes, Collect the suspended solution in a Price upon caution, and murder. (n.d.). 54 paper filter and the vacuum filter apparatus the solution Although caffeine is capable of dissolving in water by forming hydrogen bonds, the greater affinity that caffeine has for methylene chloride breaks these bonds. 8. At the same time, the insoluble cellulose components of tea separated from the solution rendering the tea concentrate, caffeine, and the new phenol anion product. Give at least three characteristics of chloroform that make it a good extracting solvent for caffeine. extraction will be performed by adding a solvent to the coffee. Tea bags. Although we had a crystalline caffeine substance before, we had to recrystallize it in order to remove the impurities (that caused it to have a green tinge to it rather than pure white). It has a density of 1.325 g/m. Introduction To filter out the drying agent a conical funnel and filter paper were used. The bottom layer was released from the stopcock and collected into a 100 mL beaker leaving behind a thin layer of methylene chloride to prevent contamination. Caffeine is soluble in water and a variety of organic solvents, and both can be used to extract caffeine from tea leaves or coffee beans. - Calcium Carbonate Graduateway.com is owned and operated by Radioplus Experts Ltd We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. - Filter Paper 2016 U6B2 Chemistry Coursework- CWH, CKS, CYL, TKM STPM 2016 REPORT OF CHEMISTRY PROJECT WORK 962/4 SMJK SAM TET (U6B2) Extraction of Caffeine NAME IC NO INDEX Kelvin Tan Kai Min 97032908-5175 SA2011/1125 Chooi Wen Han 970826-08-6013 SA2011/1121 Chooi Kai . Again, the layers separated, and the organic layer was drained in the same 50mL Erlenmeyer used before. 0000001319 00000 n USDA, Food Composition Database. qlookup=, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. The reason why salt (NaCl) was added to the aqueous layer to help break up any emulsion is because a difference in solubility must occur to separate the tannins into the aqueous layer. The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Methylene chloride was added 2 more times to assure that all of the caffeine was reacted with. Simultaneously us: [emailprotected]. Normally, hot solutions are not vacuum filtered. Using a hot plate, it was boiled for 15 minutes. 9, Cengage Learning, 2011. Next it was divided in 2 portions. Introduction 2. The green residue formed in the round-bottom flask. Retrieved from https://graduateway.com/lab-report-extraction-of-caffeine-from-tea-bags/, Biology Lab Report on the extraction of Chlorophyl, Tea Drinking Tea Drinking Event Research Paper. Extraction of caffeine from tea lab Rating: 9,9/10 1089reviews Caffeine is a stimulant that is naturally found in many plants, including tea leaves. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Title Page no. Extract the tea solution with 6 mL of dichloromethane (CH2Cl2, also called methylene chloride or DCM). Copyright 2023 service.graduateway.com. Therefore, the extraction yield was 48%. Experimental. Some chlorophyll is often extracted at the same time. The solubility of caffeine in water is 22mg/ml at 25C, 180mg/ml at 80C, and. Remedy Kombucha Good Energy. Like before, the separatory funnel was inverted a few times and it was opened so the gases would escape. leave solution was poured into a beaker. 0000001503 00000 n Experiment 2 Isolation of Caffeine from Tea AIMS Demonstrate the application of solvent extraction of natural organic compound caffeine and purification with sublimation. Max. Chloroform is an inorganic solvent, Procedure Observation What will happen if sodium sulfite is not added to the caffeine extract? Choose skilled expert on your subject and get original paper with free plagiarism Hb```5| bE8&01Lb``* bQ(f`Pggxp@\9=yLx$$R]EgL^D;7c^fbo O endstream endobj 40 0 obj 113 endobj 18 0 obj << /Type /Page /Parent 13 0 R /Resources 19 0 R /Contents 23 0 R /MediaBox [ 0 0 612 792 ] /CropBox [ 0 0 612 792 ] /Rotate 0 >> endobj 19 0 obj << /ProcSet [ /PDF /Text ] /Font << /TT2 21 0 R /TT4 24 0 R /TT6 28 0 R /TT8 29 0 R /TT10 31 0 R >> /ExtGState << /GS1 33 0 R >> /ColorSpace << /Cs6 20 0 R >> >> endobj 20 0 obj [ /ICCBased 32 0 R ] endobj 21 0 obj << /Type /Font /Subtype /TrueType /FirstChar 32 /LastChar 150 /Widths [ 320 0 0 0 0 0 0 0 359 359 0 561 260 320 260 400 500 500 500 500 500 500 500 500 500 500 260 260 0 0 0 0 0 641 641 619 740 561 520 740 740 320 0 660 0 801 699 779 561 0 580 480 580 0 0 920 0 660 0 240 0 240 0 0 0 480 561 461 561 500 301 520 561 260 0 539 260 820 561 561 580 561 340 400 279 561 500 779 520 500 461 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 221 381 381 0 500 ] /Encoding /WinAnsiEncoding /BaseFont /DFBDHL+Gatineau /FontDescriptor 22 0 R >> endobj 22 0 obj << /Type /FontDescriptor /Ascent 842 /CapHeight 656 /Descent -500 /Flags 32 /FontBBox [ -121 -248 1066 842 ] /FontName /DFBDHL+Gatineau /ItalicAngle 0 /StemV 80 /XHeight 437 /FontFile2 34 0 R >> endobj 23 0 obj << /Length 2876 /Filter /FlateDecode >> stream The solution was left to sit extract caffeine from aqueous extract of tea powder because caffeine is more soluble in. trailer << /Size 41 /Info 14 0 R /Root 17 0 R /Prev 95663 /ID[<113ce256ec8678ce243c6b9e448776e7>] >> startxref 0 %%EOF 17 0 obj << /Type /Catalog /Pages 13 0 R /Metadata 15 0 R /PageLabels 12 0 R >> endobj 39 0 obj << /S 57 /L 124 /Filter /FlateDecode /Length 40 0 R >> stream Use an organic solvent to extract the caffeine and related compounds from the water. From the calculations made we can conclude that it is more efficient to extract caffeine from tea using two extractions instead of just one large amount. mixture was transferred after it cooled. 230C The product completely melted, Transfer to Beaker with Dont Stripping this solution to dryness will take less than 5 minutes. After single extraction with 15mL, 0.178g of caffeine is in the water and 0.122g is in the dichloromethane. With a No. If a common salt like sodium carbonate was introduced to the solution, gallic acid could revert back into phenol salt: a polar, inorganic molecule that is insoluble in methylene chloride[3]. requirements? The weight of the round-bottom flask with the green residue was 54. Black tea often contains up to 5% caffeine by mass. Using these individual spikes in photon energy, the infrared spectrometer predicts the composition of the compound present. 0000006870 00000 n Since caffeine is more soluble in the organic solvent dichloromethane (CH 2 Cl 2 . This will help you determine how well your procedure worked. bottom beaker. was taken out of the boiling tea and any remaining tea in the bag was squeezed out. Lab Report: Extraction of Caffeine from Tea Bags Measure out 10ml of tea solution and add it to a centrifuge tube. Following this, a separatory funnel was used to pour the mixture in. E0r'"(``1-,w?[Z}T)?` +=A=^@d+2u|7K(}2+dHyupG$!/a@Q@|? S\~[9 AFcylSft%d2oSJK}I8`bVV3%EAJ}G9n=(bB$A0[{83b*Orp"2 . extraction of caffeine from coffee performed : james forst, sibora peca performed on submitted on abstract within this Skip to document Ask an Expert Sign inRegister Sign inRegister Home The ground solid is then treated with a solvent that removed desired components selectively. The mixture To remove the an error with the balances, or the ball flask could not have been dried enough after washing. Next, the tea Cross), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Give Me Liberty! Because Dichloromethane is denser than water, it is located at the bottom of the separatory funnel. with water, because the caffeine is soluble in it. extraction of caffeine from tea lab report introduction. We added several spoon fulls until the anhydrous calcium chloride pellets stopped clumping together. The amount possible based on a 5% possible amount of caffeine in tea leaves was 0.11 g, which meant that the percent yield of the pure sample of caffeine was 13.64%. In methylene chloride, caffeine will have a greater attraction for the organic solvent and the hydrogen bonds between caffeine and water will be broken. In this lab, we will extract caffeine from tea leaves using a solvent such as water or ethanol. separate 100ml beaker to prevent mixing the 0000005958 00000 n To start, a 150 mL beaker containing 50 mL deionized water and 2 boiling stones was prepared to dissolve 2.0 grams of sodium carbonate to react with the gallic acid in tea. A vacuum funnel using a Buchner funnel was used to remove the liquid and impurities and the remaining crystals were washed and transferred using a few drops of hexanes.